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3I91
(Plasmid #62514)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62514 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid # 26096)
  • Backbone size w/o insert (bp) 7325
  • Total vector size (bp) 7487
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CBX8
  • Alt name
    chromobox homolog 8
  • Alt name
    PDB IDs: 3I91, 5EQ0
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    162
  • GenBank ID
  • Entrez Gene
    CBX8 (a.k.a. PC3, RC1)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • TEV cleavage (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3I91 was a gift from Cheryl Arrowsmith (Addgene plasmid # 62514 ; http://n2t.net/addgene:62514 ; RRID:Addgene_62514)
  • For your References section:

    Recognition and specificity determinants of the human cbx chromodomains. Kaustov L, Ouyang H, Amaya M, Lemak A, Nady N, Duan S, Wasney GA, Li Z, Vedadi M, Schapira M, Min J, Arrowsmith CH. J Biol Chem. 2011 Jan 7;286(1):521-9. doi: 10.1074/jbc.M110.191411. Epub 2010 Nov 3. 10.1074/jbc.M110.191411 PubMed 21047797