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PurposeExpresses Beta-Lactamase protein in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62729 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-15
- Backbone size w/o insert (bp) 5637
- Total vector size (bp) 6516
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBeta-Lactamase
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SpeciesE.coli
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Insert Size (bp)792
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MutationDeletion of periplasmic signal domain
- Promoter T7
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Tag
/ Fusion Protein
- His tag (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GATCGAGATCTCGATCCCGCG
- 3′ sequencing primer TTTCAGCAAAAAACCCCTCAAGACC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Beta-Lactamase was a gift from Niels Geijsen (Addgene plasmid # 62729 ; http://n2t.net/addgene:62729 ; RRID:Addgene_62729) -
For your References section:
Efficient Intracellular Delivery of Native Proteins. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V, Lebbink RJ, Rehmann H, Geijsen N. Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028. 10.1016/j.cell.2015.03.028 PubMed 25910214