SP-Cas9
(Plasmid #62731)

• Purpose: Expresses SP-Cas9 protein in bacterial cells
• Depositing Lab: Niels Geijsen
• Publication: D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.

Backbone

• Vector backbone: pET-15
• Backbone size w/o insert (bp): 5562
• Total vector size (bp): 9822
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SP-CAS9
• Alt name: CAS9 from Streptococcus pyogenes serotype M1
• Species: Streptococcus pyogenes serotype M1
• Insert Size (bp): 4179
• Mutation: Codon optimazed for e.coli
• GenBank ID: GI:81533697 Q99ZW2
• Promoter: T7
• Tags / Fusion Proteins:
  • NLS (C terminal on insert)
  • His tag (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GATCGAGATCTCGATCCCGCG
• 3′ sequencing primer: TTTCAGCAAAAAACCCCTCAAGACC

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry