|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62803||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1(-) (modified)
Backbone manufacturerMinczuk Laboratory
- Backbone size (bp) 5343
Modifications to backboneComplete pcDNA3.1(-) with intact multiple cloning site. Neomycin resistance cassette replaced with mCherry, using Gibson Assembly.
Vector typeMammalian Expression
- Promoter CMV
Selectable markersmCherry fluorescence
Growth in Bacteria
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcmCherry 3.1(-) was a gift from Michal Minczuk (Addgene plasmid # 62803 ; http://n2t.net/addgene:62803 ; RRID:Addgene_62803)