Blue opsin mcherry ---- pcDNA3.1
PurposeBlue opsin tagged with mcherry on the C terminus
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62932||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 7181
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameblue opsin
Insert Size (bp)1071
- Promoter CMV
/ Fusion Protein
- mCherry (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Blue opsin can be excised by cutting with EcoR1 and NotI. There is an internal XbaI in the blue opsin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Blue opsin mcherry ---- pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 62932 ; http://n2t.net/addgene:62932 ; RRID:Addgene_62932)
For your References section:Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension. Karunarathne WK, Giri L, Kalyanaraman V, Gautam N. Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 10.1073/pnas.1220697110. Epub 2013 Mar 11. 10.1073/pnas.1220697110 PubMed 23479634