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pAAV CTG 700x
(Plasmid #63087)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63087 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    low Amp
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CTG REPEATS
  • Species
    Synthetic
  • Mutation
    CTG 700x that is the mutation found in Myotonic Dystrophic type 1
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.

This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV CTG 700x was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 63087 ; http://n2t.net/addgene:63087 ; RRID:Addgene_63087)