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pAAV CTG 700x Notes

Addgene Diagnostic Test

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 63087 with the following enzymes: BamHI and BamHI+NheI

We expected the linearized plasmid to be 7.5kb and the double digest to give bands of 2.2kb and 5.3kb. We see the expected results, albeit with some additional degradation products.

Addgene Diagnostic Test

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 63087 with the following enzymes: EcoRI-HF

We expected the digest to give bands of 2.9kb and 4.7kb. We see the expected results, albeit with some additional degradation products.