pSPble
(Plasmid
#63561)
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PurposeBleomycin drug selection marker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63561 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSP72
- Total vector size (bp) 3114
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Vector typeLeishmania Expression
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Selectable markersBleomycin or Phlemycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBleomycin drug selection marker
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Alt nameble
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Insert Size (bp)680
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH I (not destroyed)
- 3′ cloning site Bgl II (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector can be used as the PCR template to generate the ble expression cassette donor for gene disruption with CRISPR-CAS9
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSPble was a gift from Greg Matlashewski (Addgene plasmid # 63561 ; http://n2t.net/addgene:63561 ; RRID:Addgene_63561) -
For your References section:
CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani. Zhang WW, Matlashewski G. MBio. 2015 Jul 21;6(4). pii: e00861-15. doi: 10.1128/mBio.00861-15. 10.1128/mBio.00861-15 PubMed 26199327
