Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pMT-DREDD-V5-His
(Plasmid #63749)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63749 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMT-V5-His
  • Backbone manufacturer
    lifetechnologies
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 5011
  • Modifications to backbone
    none
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Death related ced-3/Nedd2-like caspase isoform E
  • Alt name
    Dredd
  • Alt name
    Dcp-2/Dredd
  • Species
    D. melanogaster (fly)
  • GenBank ID
    NM_057901.4 NP_477249.3
  • Entrez Gene
    Dredd (a.k.a. Dmel_CG7486, CASP8_DROME, CG7486, DCP-2, DCP-2/DREDD, DCP2, DREDD, Dcp-2, Dcp-2/Dredd, Dcp2, Dedd, Dmel\CG7486, Dredd/DCP-2, EG:115C2.9, Redd, anon-1BCa, dDredd, dcp-2/dredd, dredd)
  • Promoter pMT
  • Tag / Fusion Protein
    • V5-6XHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CATCTCAGTGCAACTAAA
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-DREDD-V5-His was a gift from Cyril Gueydan (Addgene plasmid # 63749 ; http://n2t.net/addgene:63749 ; RRID:Addgene_63749)