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M_ST1n_VPR
(Plasmid #63799)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63799 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.3 TOPO
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ST1-dCas9-VPR
  • Alt name
    VP64-p65-Rta
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    4922
  • Promoter CMV
  • Tag / Fusion Protein
    • VPR (VP64-p65-Rta) (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    addgene #48669
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cas9 insert contains K604R, D839A and N863A mutations relative to reference sequence WP_010922251.1. Depositor states that these mutations should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M_ST1n_VPR was a gift from George Church (Addgene plasmid # 63799 ; http://n2t.net/addgene:63799 ; RRID:Addgene_63799)
  • For your References section:

    Highly efficient Cas9-mediated transcriptional programming. Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. 10.1038/nmeth.3312 PubMed 25730490