PPDK
(Plasmid #64037)

• Purpose: Expression of Pyruvate phosphate dikinase (PPDK) in E. coli
• Depositing Lab: Vern Schramm
• Publication: Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. doi: 10.1021/ac8026433.

Backbone

• Vector backbone: pACYC184
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: JM101 was used for protein expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: pyruvate phosphate dikinase
• Alt name: PPDK
• Alt name: pyruvate, orthophosphate dikinase
• Alt name: ATP:pyruvate, phosphate phosphotransferase
• Species: Bacteroides symbiosus
• Tag / Fusion Protein:
  • none

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Professor Debra Dunaway-Mariano, Department of Chemistry, University of New Mexico

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Written permission was obtained from Prof. Dunaway-Mariano of University of New Mexico, allowing the deposit this plasmid to Addgene.
Pacolyko, D. J., Carroll, L. J., Martin, B. M., Babbitt, P. C., and Dunaway-Mariano, D. (1990) Biochemistry 29, 10757−10765.