|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64255||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9289
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
- 3′ cloning site BbsI (destroyed during cloning)
- 5′ sequencing primer ACTATCATATGCTTACCGTAAC (Common Sequencing Primers)
Terms and Licenses
Confirmed by the Mendenhall and Myers labs to Flag epitope tag the endogenous human GABPA gene when pooled with plasmids PX548_GABPA_1 and pFETCH_GABPA and transfected into human cells. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PX458_GABPA_2 was a gift from Eric Mendenhall & Richard M. Myers (Addgene plasmid # 64255 ; http://n2t.net/addgene:64255 ; RRID:Addgene_64255)
For your References section:CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM. Genome Res. 2015 Sep 9. 10.1101/gr.193540.115 PubMed 26355004