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6691 Bicistronic_ires_puro
(Plasmid #64335)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64335 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Migr1
  • Backbone manufacturer
    Theodora Agalioti
  • Modifications to backbone
    HindIII and HpaI digest
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses

Depositor Comments

This construct was created in G. Stathopoulos lab in Patras, Greece, by Theodora Agalioti; The backbone is derived from MigR1 vector to which a partial IRES followed by puromycin selection gene derived from pPY_CAG_CRE_IP plasmid was inserted

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6691 Bicistronic_ires_puro was a gift from Georgios Stathopoulos (Addgene plasmid # 64335 ; http://n2t.net/addgene:64335 ; RRID:Addgene_64335)
  • For your References section:

    Mutant KRAS promotes malignant pleural effusion formation. Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M, Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, Psallidas I, Marazioti A, Stathopoulos GT. Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205. 10.1038/ncomms15205 PubMed 28508873