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pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
(Plasmid #119816)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119816 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLentipuro3/TO/V5-DEST
  • Backbone size w/o insert (bp) 8116
  • Total vector size (bp) 10489
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Luciferase
  • Alt name
    Firefly luciferase
  • Species
    Photinus pyralis
  • Insert Size (bp)
    2373
  • Mutation
    Stop codon removed in the frame of the V5 frame vector
  • Promoter CMV/TO
  • Tags / Fusion Proteins
    • EGFP (N terminal on insert)
    • V5 (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Puromycin
  • Species
    Streptomyces alboniger
  • Insert Size (bp)
    600
  • Promoter Human Phosphoglycerate kinase

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Looking for an anti-GFP antibody? Check out our recombinant mouse monoclonal antibody: https://www.addgene.org/180084/.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase was a gift from Ethan Abel (Addgene plasmid # 119816 ; http://n2t.net/addgene:119816 ; RRID:Addgene_119816)
  • For your References section:

    A surgical orthotopic approach for studying the invasive progression of human bladder cancer. Lorenzatti Hiles G, Cates AL, El-Sawy L, Day KC, Broses LJ, Han AL, Briggs HL, Emamdjomeh A, Chou A, Abel EV, Liebert M, Palmbos PL, Udager AM, Keller ET, Day ML. Nat Protoc. 2019 Mar;14(3):738-755. doi: 10.1038/s41596-018-0112-8. 10.1038/s41596-018-0112-8 PubMed 30683938