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AR-V7-pcw107-V5
(Plasmid #64636)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64636 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcw107 (V5)
  • Backbone manufacturer
    John Doench/Kathleen Ottina
  • Backbone size w/o insert (bp) 9240
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AR (transcript variant 1, splice isoform)
  • Alt name
    NM_000044.3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1932
  • Mutation
    splice isoform *
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Promoter PGK
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Charles Sawyers Lab, MSK/HHMI
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

GCTG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-P1-V5

*Addgene sequence shows that there is an L57Q mutation in AR-V7 relative to NCBI sequences. This mutation was in the original Sawyers lab clone and is not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AR-V7-pcw107-V5 was a gift from David Sabatini & Kris Wood (Addgene plasmid # 64636 ; http://n2t.net/addgene:64636 ; RRID:Addgene_64636)
  • For your References section:

    Systematic identification of signaling pathways with potential to confer anticancer drug resistance. Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN, Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, McDonnell DP, Sabatini DM, Wood KC. Sci Signal. 2014 Dec 23;7(357):ra121. doi: 10.1126/scisignal.aaa1877. 10.1126/scisignal.aaa1877 PubMed 25538079