|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64710||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3931
- Total vector size (bp) 4384
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Copy numberHigh Copy
Gene/Insert nameStaphylococcus aureus guide RNA
- Promoter hU6
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSaGuide was a gift from Kiran Musunuru (Addgene plasmid # 64710 ; http://n2t.net/addgene:64710 ; RRID:Addgene_64710)