RhoC FLARE.sc mCer, mVenus - wt
PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65071||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 7888
Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRBD, mCerulean, mVenus, RhoC
Insert Size (bp)2688
MutationT153M in mVenus
- Promoter CMV, p10
- Cloning method Unknown
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RhoC FLARE.sc mCer, mVenus - wt was a gift from Klaus Hahn (Addgene plasmid # 65071 ; http://n2t.net/addgene:65071 ; RRID:Addgene_65071)
For your References section:A RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells. Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L. PLoS One. 2013 Nov 5;8(11):e79877. doi: 10.1371/journal.pone.0079877. eCollection 2013. PONE-D-13-18183 [pii] PubMed 24224016
Map uploaded by the depositor.