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AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
(Plasmid #65454)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65454 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-MCS
  • Backbone manufacturer
    Stratagene
  • Modifications to backbone
    A fragment containing: the hSyn1 promoter-a (Frt-Stop-Frt) cassette-a FLEX cassette with Lox71/LoxJTZ17 sites flanking (ChR2H134R-EYFP)-WPRE-bGHpA were swapped in to replace the CMV promoter-MCS-hGHpA sequence. Total insert size including the two ITRs is 4443 bp.
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ChR2(H134R)-EYFP
  • Alt name
    channelrhodopsin-2 variant H134R-EYFP
  • Species
    C. reinhardtii
  • Insert Size (bp)
    1671
  • Mutation
    H134R variant
  • Promoter hSyn1
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (destroyed during cloning)
  • 3′ cloning site Not1 (destroyed during cloning)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA was a gift from Hongkui Zeng (Addgene plasmid # 65454)
  • For your References section:

    Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, Zeng H. Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022. 10.1016/j.neuron.2015.02.022 PubMed 25741722