AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
(Plasmid #65454)

• Purpose: Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases
• Depositing Lab: Hongkui Zeng
• Publication: Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.

Backbone

• Vector backbone: pAAV-MCS
• Backbone manufacturer: Stratagene
• Modifications to backbone: A fragment containing: the hSyn1 promoter-a (Frt-Stop-Frt) cassette-a FLEX cassette with Lox71/LoxJTZ17 sites flanking (ChR2H134R-EYFP)-WPRE-bGHpA were swapped in to replace the CMV promoter-MCS-hGHpA sequence. Total insert size including the two ITRs is 4443 bp.
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ChR2(H134R)-EYFP
• Alt name: channelrhodopsin-2 variant H134R-EYFP
• Species: C. reinhardtii
• Insert Size (bp): 1671
• Mutation: H134R variant
• Promoter: hSyn1
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (destroyed during cloning)
• 3′ cloning site: Not1 (destroyed during cloning)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry