Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, Zeng H
Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022. PubMed Article
Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, Zeng H
Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50363 | AAV phSyn1(S)-DreO-bGHpA | Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter |
| 50364 | AAV phSyn1(S)-FlpE-bGHpA | Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter |
| 51507 | AAV pmSyn1-EBFP-Cre | Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter |
| 51669 | AAV phSyn1(S)-FlpO-bGHpA | Can be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter. |
| 61570 | Pvalb-2A-Cre targeting vector | Target the Cre recombinase gene to the stop codon of the mouse Pvalb gene |
| 61571 | Pvalb-2A-Dre Flp-in replacement vector | Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon |
| 61572 | Pvalb-2A-Flpo Flp-in replacement vector | Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon |
| 61573 | Rasgrf2-2A-dCre targeting vector | Target a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene |
| 61574 | Slc17a7-IRES2-Cre targeting vector | Target the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene |
| 61575 | Snap25-LSL-2A-GFP targeting vector | Target a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene |
| 61576 | Ai62(TITL-tdT) Flp-in replacement vector | Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus |
| 61577 | Ai65(RCFL-tdT) targeting vector | Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus |
| 61578 | Ai66(RCRL-tdT) targeting vector | Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus |
| 61579 | Ai95(RCL-GCaMP6f) targeting vector | Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus |
| 61580 | TIGRE docking site targeting vector | Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange |
| 65429 | AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA | Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases |
| 65449 | AAV pTRE-FLEX-EGFP-WPRE-bGHpA | Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner |
| 65453 | AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA | Can be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases |
| 65454 | AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA | Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases |
| 65455 | AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA | Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases |
| 65458 | AAV pCAG-FLEX2-tTA2-WPRE-bGHpA | Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner |