AAV pTRE-FLEX-EGFP-WPRE-bGHpA
(Plasmid #65449)

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner
Depositing Lab

Hongkui Zeng
Publication

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022. ( How to cite )
Sequence Information
- Sequences (2)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	65449	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pAAV-MCS
(Search Vector Database)
Backbone manufacturer
Stratagene
Modifications to backbone
A fragment containing the tetO promoter-FLEX-EGFP-WPRE-bGHpA was swapped in to replace the CMV promoter-MCS-hGHpA sequence. Total insert size including the two ITRs is 2803 bp.
Vector type
AAV

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
30°C
Growth Strain(s)
Stbl2
Copy number
Unknown

Gene/Insert

Gene/Insert name
EGFP
Insert Size (bp)
720
Promoter tetO

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site Not1 (not destroyed)
3′ cloning site Not1 (not destroyed)
5′ sequencing primer none
3′ sequencing primer none

(Common Sequencing Primers)

Resource Information

Terms and Licenses
Articles Citing this Plasmid
- 2 References

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

AAV pTRE-FLEX-EGFP-WPRE-bGHpA was a gift from Hongkui Zeng (Addgene plasmid # 65449 ; http://n2t.net/addgene:65449 ; RRID:Addgene_65449)
For your References section:

Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, Zeng H. Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022. 10.1016/j.neuron.2015.02.022 PubMed 25741722

Map uploaded by the depositor.

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AAV pTRE-FLEX-EGFP-WPRE-bGHpA (Plasmid #65449)