AAV pTRE-FLEX-EGFP-WPRE-bGHpA
(Plasmid #65449)

• Purpose: Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner
• Depositing Lab: Hongkui Zeng
• Publication: Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	65449	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pAAV-MCS
  (Search Vector Database)
• Backbone manufacturer: Stratagene
• Modifications to backbone: A fragment containing the tetO promoter-FLEX-EGFP-WPRE-bGHpA was swapped in to replace the CMV promoter-MCS-hGHpA sequence. Total insert size including the two ITRs is 2803 bp.
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP
• Insert Size (bp): 720
• Promoter: tetO

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • genOway Notice of RIghts
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 2 References