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dg264_pENTR_ChronosCel
(Plasmid #66102)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66102 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57-Kan
  • Total vector size (bp) 3927
  • Modifications to backbone
    The insert in this vector is flanked by attL1 and attL2 sites and is suitable for LR recombination reactions (Gateway system).
  • Vector type
    Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Chronos
  • Alt name
    ChronosCel
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1128

Resource Information

Depositor Comments

This construct was produced through de novo gene synthesis.

Chronos amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dg264_pENTR_ChronosCel was a gift from Dominique Glauser (Addgene plasmid # 66102)
  • For your References section:

    Dual Color Neural Activation and Behavior Control with Chrimson and CoChR in Caenorhabditis elegans. Schild LC, Glauser DA. Genetics. 2015 May 28. pii: genetics.115.177956. 10.1534/genetics.115.177956 PubMed 26022242