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pGL4.10-VEGFprom(-1000 to -500)
(Plasmid #66129)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66129 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL4.10
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4242
  • Total vector size (bp) 4695
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vascular Endothelial Growth Factor -A promoter region
  • Alt name
    VEGF
  • Alt name
    VEGFA
  • Species
    H. sapiens (human)
  • Entrez Gene
    VEGFA (a.k.a. MVCD1, VEGF, VPF)
  • Promoter VEGF -500 to -1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer TTAAGGTACCCCAGTCCCAAATATGTAGCTGTT
  • 3′ sequencing primer TTAAAAGCTTCCAAGGTTCACAGCCTGAAA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.10-VEGFprom(-1000 to -500) was a gift from David Mu (Addgene plasmid # 66129 ; http://n2t.net/addgene:66129 ; RRID:Addgene_66129)
  • For your References section:

    Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome. Wood LW, Cox NI, Phelps CA, Lai SC, Poddar A, Talbot C Jr, Mu D. Sci Rep. 2016 Feb 25;6:19857. doi: 10.1038/srep19857. 10.1038/srep19857 PubMed 26912193