This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed December 24th & 25th and December 31st & January 1st. For details, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pBS-IT6
(Plasmid #66870)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66870 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Inntag 6
  • Alt name
    PDB_ID 1WKX
  • Species
    Hevea brasiliensis
  • Insert Size (bp)
    129

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GTTGTG TGGAATTGTGAGCG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-IT6 was a gift from Marti Aldea (Addgene plasmid # 66870 ; http://n2t.net/addgene:66870 ; RRID:Addgene_66870)
  • For your References section:

    Inntags: small self-structured epitopes for innocuous protein tagging. Georgieva MV, Yahya G, Codo L, Ortiz R, Teixido L, Claros J, Jara R, Jara M, Iborra A, Gelpi JL, Gallego C, Orozco M, Aldea M. Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556. 10.1038/nmeth.3556 PubMed 26322837