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LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
(Plasmid #67277)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67277 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Amp
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 16500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
  • Insert Size (bp)
    1500
  • Promoter CAG
  • Tag / Fusion Protein
    • red flourescent gene katushka linked to puro through a E2A site (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site Asis (unknown if destroyed)
  • 5′ sequencing primer gctggttgttgtgctgtctc
  • 3′ sequencing primer cagacccttgccctggtg
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
  • Insert Size (bp)
    5700
  • Promoter CAG
  • Tag / Fusion Protein
    • mTQ2 blue fluorescent (N terminal on backbone)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asis (unknown if destroyed)
  • 3′ cloning site MluI (unknown if destroyed)
  • 5′ sequencing primer gatcacatggtcctgctg
  • 3′ sequencing primer acaccgaggagaatgtcaag
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR was a gift from Jannik Elverløv-Jakobsen (Addgene plasmid # 67277 ; http://n2t.net/addgene:67277 ; RRID:Addgene_67277)