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Villinpromoter-blue-FlpOERT2
(Plasmid #67278)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67278 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTQ2=mturquoise2 blue fluorescent gene
  • Species
    blue fluorescent gene
  • Insert Size (bp)
    800
  • Promoter Villin
  • Tag / Fusion Protein
    • linked to FlpO through an P2A ribosomal skipping site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (unknown if destroyed)
  • 3′ cloning site Bstz17I (unknown if destroyed)
  • 5′ sequencing primer tagaggttggactacagtgg
  • 3′ sequencing primer tcttgatgtcgctgaacctg
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Villinpromoter-blue-FlpOERT2 was a gift from Jannik Elverløv-Jakobsen (Addgene plasmid # 67278 ; http://n2t.net/addgene:67278 ; RRID:Addgene_67278)