PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67509||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Vector backbonepCAGGS vector
- Backbone size w/o insert (bp) 4900
- Total vector size (bp) 6406
Growth in Bacteria
Growth instructionsDH5alpha is also a suitable growth strain.
Copy numberHigh Copy
Gene/Insert nameFusion Glycoprotein type E
SpeciesG. gallus (chicken), B. taurus (bovine); Vesicular stomatitis Indiana virus, Rabies virus, Cytomegalovirus, Simian virus 40, Oryctolagus cuniculus
Insert Size (bp)1515
- Promoter CAGGS
/ Fusion Protein
- Fusion protein of Vesicular stomatitis Indiana virus and Rabies virus (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer Unknown
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAGGS-FuG-E was a gift from Kazuto Kobayashi (Addgene plasmid # 67509 ; http://n2t.net/addgene:67509 ; RRID:Addgene_67509)
For your References section:Improved transduction efficiency of a lentiviral vector for neuron-specific retrograde gene transfer by optimizing the junction of fusion envelope glycoprotein. Kato S, Kobayashi K, Kobayashi K. J Neurosci Methods. 2014 Apr 30;227:151-8. doi: 10.1016/j.jneumeth.2014.02.015. Epub 2014 Mar 5. 10.1016/j.jneumeth.2014.02.015 PubMed 24613797