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pCAGGS-FuG-B
(Plasmid #67523)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67523 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGGS vector
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 6421
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    DH5alpha is also a suitable growth strain.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fusion Glycoprotein type B
  • Alt name
    FuG-B
  • Species
    G. gallus (chicken), B. taurus (bovine); Vesicular stomatitis Indiana virus, Rabies virus, Cytomegalovirus, Simian virus 40, Oryctolagus cuniculus
  • Insert Size (bp)
    1530
  • Promoter CAGGS
  • Tag / Fusion Protein
    • Fusion protein of Vesicular stomatitis Indiana virus and Rabies virus (C terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Fusion glycoproteins are composed of parts of rabies virus glycoprotein (RV-G) and vesicular stomatitis virus glycoprotein (VSV-G). In three glycoproteins including FuG-B, FuG-C, and FuG-E, the sequences of RV-G are derived from the challenged virus standard (CVS) strain of rabies virus, whereas those in FuG-B2 are from Pasteur virus (PV) strain of the virus. The CVS RV-G cDNA was provided by Dr. K. Morimoto at Yasuda Women’s University (Hiroshima, Japan) (ref: MORIMOTO, K., HOOPER, D.C., CARBAUGH, H., FU, Z.F., KOPROWSKI, H., and DIETZSCHOLD, B. Rabies virus quasispecies: implications for pathogenesis. Proc. Natl. Acad. Sci. USA 95, 3152-3156, 1998), and the PV RV-G cDNA was chemically synthesized on the basis of the GenBank data (accession number: GU992322). The VSV-G cDNA was provided by Dr. A. Nienhuis at St. Jude Children’s Research Hospital.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-FuG-B was a gift from Kazuto Kobayashi (Addgene plasmid # 67523 ; http://n2t.net/addgene:67523 ; RRID:Addgene_67523)
  • For your References section:

    A lentiviral strategy for highly efficient retrograde gene transfer by pseudotyping with fusion envelope glycoprotein. Kato S, Kobayashi K, Inoue K, Kuramochi M, Okada T, Yaginuma H, Morimoto K, Shimada T, Takada M, Kobayashi K. Hum Gene Ther. 2011 Feb;22(2):197-206. doi: 10.1089/hum.2009.179. Epub 2011 Jan 27. 10.1089/hum.2009.179 PubMed 20954846