pAAV-EF1a-tdTomato-WPRE-pGHpA
(Plasmid #67527)

• Purpose: "Expresses the fluorescent protein tdTomato"
• Depositing Lab: Botond Roska
• Publication: Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi: 10.1126/science.aab1687.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	67527	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
  (Search Vector Database)
• Backbone manufacturer: karl deisseroth
• Total vector size (bp): 6775
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tandem dimer tomato
• Alt name: tdTomato
• Insert Size (bp): 1428
• Promoter: EF1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Terms and Licenses:
  • UBMTA
  • Clontech Limited Use Label License
• Article Citing this Plasmid:
  • 1 Reference