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Purpose"Expresses the fluorescent protein tdTomato"
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 67527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
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Backbone manufacturerkarl deisseroth
- Total vector size (bp) 6775
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametandem dimer tomato
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Alt nametdTomato
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Insert Size (bp)1428
- Promoter EF1a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-tdTomato-WPRE-pGHpA was a gift from Botond Roska (Addgene plasmid # 67527 ; http://n2t.net/addgene:67527 ; RRID:Addgene_67527) -
For your References section:
PRESYNAPTIC NETWORKS. Single-cell-initiated monosynaptic tracing reveals layer-specific cortical network modules. Wertz A, Trenholm S, Yonehara K, Hillier D, Raics Z, Leinweber M, Szalay G, Ghanem A, Keller G, Rozsa B, Conzelmann KK, Roska B. Science. 2015 Jul 3;349(6243):70-4. doi: 10.1126/science.aab1687. 10.1126/science.aab1687 PubMed 26138975