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pFUGW-hGtACR1-EYFP
(Plasmid #67795)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67795 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hGtACR1
  • Alt name
    GtACR1
  • Alt name
    Guillardia theta anion channelrhodopsin (ACR)
  • Species
    Guillardia theta
  • Insert Size (bp)
    1613
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Resource Information

Depositor Comments

Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site, in the pFUGW vector backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFUGW-hGtACR1-EYFP was a gift from John Spudich (Addgene plasmid # 67795)
  • For your References section:

    Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics. Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL. Science. 2015 Jun 25. pii: aaa7484. 10.1126/science.aaa7484 PubMed 26113638