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pHO4d-Cas9
(Plasmid #67881)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67881 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHO4D
  • Backbone size w/o insert (bp) 5712
  • Total vector size (bp) 9841
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    Streptococcus pyrogenes M1
  • Insert Size (bp)
    4152
  • GenBank ID
    bp 854751 to 858854 of AE004092.2
  • Promoter T7
  • Tags / Fusion Proteins
    • His6 (C terminal on insert)
    • T7 epitope (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer GCGTAGAGGATCGAGATC
  • 3′ sequencing primer CTTCCTTTCGGGCTTTGTTAGCAGCCGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The insert was cloned into this his6 expression vector from AddGene plasmid #42251
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Transform into an appropriate protein expression E. coli strain like BL21 lambda DE3 to purify Cas9

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHO4d-Cas9 was a gift from Michael Nonet (Addgene plasmid # 67881 ; http://n2t.net/addgene:67881 ; RRID:Addgene_67881)
  • For your References section:

    Landscape of target:guide homology effects on Cas9-mediated cleavage. Fu BX, Hansen LL, Artiles KL, Nonet ML, Fire AZ. Nucleic Acids Res. 2014 Dec 16;42(22):13778-87. doi: 10.1093/nar/gku1102. Epub 2014 Nov 15. 10.1093/nar/gku1102 PubMed 25399416