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GFP S2TAG
(Plasmid #68296)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68296 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP S2TAG
  • Species
    Synthetic
  • Tag / Fusion Protein
    • His (C terminal on insert)

Resource Information

Depositor Comments

Encodes reporter protein to assess efficiency of phosphoserine incorporation at TAG codons.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP S2TAG was a gift from Jesse Rinehart (Addgene plasmid # 68296 ; http://n2t.net/addgene:68296 ; RRID:Addgene_68296)
  • For your References section:

    A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. 10.1038/ncomms9130 PubMed 26350500