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A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
PubMed Article

Plasmids from Article

ID Plasmid  
68283SepOTSβ
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68284SepOTSγ
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68285SepOTSδ
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68286SepOTSε
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68287SepOTSζ
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68288SepOTSη
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68289SepOTSθ
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68290SepOTSι
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68291SepOTSκ
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68292SepOTSλ (recommended)
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68294SepOTSν
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68295GFP E17TAG
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68296GFP S2TAG
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68297GFP Q157TAG
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68298GFP S2TAG/E17TAG
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68299GFP E17TAG/Q157TAG
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68300MBP-MEK1
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68301MBP-MEK1 S218TAG
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68302MBP-MEK1 S222TAG
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68305Beta lactamase S68TAG
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68306Strain: C321.ΔA
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68307SupD
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69118E17TAG GFP zeo resistance
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