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pUC_sgRNA
(Plasmid #68710)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68710 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUCAmp
  • Backbone manufacturer
    Blue Heron
  • Backbone size (bp) 3150
  • Modifications to backbone
    tracrRNA sequence (82 bp) was custom-made synthesized by Blue Heron and inserted into the cloning site of pUCAmp vector
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer M13_forward20_primer and M13_pUC_fwd_primer
  • 3′ sequencing primer M13_reverse_primer and M13_pUC_rev_primer
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We purchased this custom-made plasmid from Blue Heron Biotech, LLC (Bothell, WA 98021 USA)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC_sgRNA was a gift from Roberto Docampo (Addgene plasmid # 68710 ; http://n2t.net/addgene:68710 ; RRID:Addgene_68710)
  • For your References section:

    CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment. Lander N, Li ZH, Niyogi S, Docampo R. MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15. 10.1128/mBio.01012-15 PubMed 26199333