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PurposeFor making transgenic fish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepExpress1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namebeta-actin promoter
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)11000
Gene/Insert 2
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Gene/Insert nameloxp-stop-codon-loxp-dsred
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
bactin2:loxP-STOP-loxP-DsRed-express was a gift from David Traver (Addgene plasmid # 68797 ; http://n2t.net/addgene:68797 ; RRID:Addgene_68797) -
For your References section:
Haematopoietic stem cells derive directly from aortic endothelium during development. Bertrand JY, Chi NC, Santoso B, Teng S, Stainier DY, Traver D. Nature. 2010 Mar 4;464(7285):108-11. doi: 10.1038/nature08738. Epub 2010 Feb 14. 10.1038/nature08738 PubMed 20154733