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pcDNA3 p53 S15A
(Plasmid #69004)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69004 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p53
  • Species
    H. sapiens (human)
  • Mutation
    S15A
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 p53 S15A was a gift from David Meek (Addgene plasmid # 69004 ; http://n2t.net/addgene:69004 ; RRID:Addgene_69004)
  • For your References section:

    Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters. Loughery J, Cox M, Smith LM, Meek DW. Nucleic Acids Res. 2014 Jul;42(12):7666-80. doi: 10.1093/nar/gku501. Epub 2014 Jun 13. 10.1093/nar/gku501 PubMed 24928858