pDonor_hU6
(Plasmid #69312)

• Purpose: sgRNA scaffold and human U6 promoter
• Depositing Lab: Andrea Ventura
• Publication: Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083.

Backbone

• Vector backbone: pBluescript KS+
• Backbone size w/o insert (bp): 2958
• Total vector size (bp): 3396
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hU6 promoter and sgRNA scaffold flanked by BbsI sites
• Insert Size (bp): 415
• Promoter: human U6 promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • pDonor Human u6
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry