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pDonor_mU6
(Plasmid #69350)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69350 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Topo Blunt II
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 4027
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mU6 promoter and sgRNA scaffold flanked by BbsI sites
  • Insert Size (bp)
    488
  • Promoter mU6 promoter

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDonor_mU6 was a gift from Andrea Ventura (Addgene plasmid # 69350 ; http://n2t.net/addgene:69350 ; RRID:Addgene_69350)
  • For your References section:

    Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries. Vidigal JA, Ventura A. Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083. 10.1038/ncomms9083 PubMed 26278926