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pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC
(Plasmid #69587)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69587 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRRL
  • Backbone size w/o insert (bp) 7765
  • Total vector size (bp) 11150
  • Modifications to backbone
    hygromycin resistance
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p53-L344P
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1179
  • Mutation
    p53 L344P mutation that prevents dimerization and tetramerization and 7 silent mutations in p53 DNA to prevent targeting by shRNA at positions C777T, T778A, C779G, A781T, G782C, T786A, T789C
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter EF1alpha
  • Tags / Fusion Proteins
    • mKate2 (C terminal on insert)
    • split mVenus - C term (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3. This is tagged with the C-terminus of a split mVenus. The L344P mutation prevents tetramerization and dimerization of p53.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC was a gift from Galit Lahav (Addgene plasmid # 69587 ; http://n2t.net/addgene:69587 ; RRID:Addgene_69587)
  • For your References section:

    Constant rate of p53 tetramerization in response to DNA damage controls the p53 response. Gaglia G, Lahav G. Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168. PubMed 25344068