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Browse > Connie Cepko > Matsuda et al > pCAG-Flpe
Plasmid 13787: pCAG-Flpe

  • Flpe

  • enhanced form of Flp recombinase

  • 1332

  • S. cerevisiae (budding yeast)

  • Myc

  • C terminal on insert

  • pCAGEN
    (Search Vector Database)

  • Mammalian Expression

  • 4779

  • EcoRI

  • No

  • NotI

  • No

  • pCAG-F List of Sequencing Primers

  • Bglob-pA-R

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • The coding sequence of Flpe was amplified by PCR using pCMVscript-FlpeERT2 (Hunter et al. Genesis 41, 99-109 (2005)) obtained from Dr. S. Dymecki (Harvard Medical School) as a template.

  • View sequences (3)

  • View map

  • Notes from Addgene (1)

  • Connie Cepko

  • MTA

Comments: 

Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation. Myc-tag was added at the C-terminus of Flpe.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda et al (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13787" in your Materials and Methods section.

Price: $65.00

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