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pTC223
(Plasmid #70019)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70019 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLSLR
  • Backbone manufacturer
    n/a
  • Total vector size (bp) 17961
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nuclease (Cas9/sgRNA) + Donor + GVR
  • Species
    Solanum lycopersicum
  • Insert Size (bp)
    11728

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTC223 was a gift from Daniel Voytas (Addgene plasmid # 70019 ; http://n2t.net/addgene:70019 ; RRID:Addgene_70019)
  • For your References section:

    High-frequency, precise modification of the tomato genome. Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF. Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9. 10.1186/s13059-015-0796-9 [pii] PubMed 26541286