p045
(Plasmid
#70057)
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PurposeCo-expresses Syt1-SNARE37aa-linker complex with pDC184
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepETDuet-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameC2AB-linker-SNAP-25_C
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1149
- Promoter T7
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameSNAP-25_N
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SpeciesR. norvegicus (rat)
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Insert Size (bp)231
- Promoter T7
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Tag
/ Fusion Protein
- No tags
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p045 was a gift from Axel Brunger (Addgene plasmid # 70057 ; http://n2t.net/addgene:70057 ; RRID:Addgene_70057) -
For your References section:
Architecture of the synaptotagmin-SNARE machinery for neuronal exocytosis. Zhou Q, Lai Y, Bacaj T, Zhao M, Lyubimov AY, Uervirojnangkoorn M, Zeldin OB, Brewster AS, Sauter NK, Cohen AE, Soltis SM, Alonso-Mori R, Chollet M, Lemke HT, Pfuetzner RA, Choi UB, Weis WI, Diao J, Sudhof TC, Brunger AT. Nature. 2015 Sep 3;525(7567):62-7. doi: 10.1038/nature14975. Epub 2015 Aug 17. 10.1038/nature14975 PubMed 26280336
Map uploaded by the depositor.
