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pGME
(Plasmid #71252)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71252 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGM
  • Backbone manufacturer
    Cockerill lab, Addgene plasmid 71250
  • Total vector size (bp) 6766
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GM-CSF enhancer
  • Alt name
    colony stimulating factor 2 (granulocyte-macrophage)
  • Species
    H. sapiens (human)
  • Entrez Gene
    CSF2 (a.k.a. CSF, GMCSF)
  • Promoter GM-CSF
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Note that the 717-bp GM-CSF enhancer contains an extra G at position 118 from the sequence published in Cockerill et al. Proc Natl Acad Sci USA. 1993;90:2466–2470.

JM109 are the cells used by the author for pXPG and its derivatives.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGME was a gift from Peter Cockerill (Addgene plasmid # 71252 ; http://n2t.net/addgene:71252 ; RRID:Addgene_71252)
  • For your References section:

    The human granulocyte-macrophage colony-stimulating factor gene is autonomously regulated in vivo by an inducible tissue-specific enhancer. Cockerill PN, Bert AG, Roberts D, Vadas MA. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15097-102. PubMed 10611344