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pGM
(Plasmid #71250)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71250 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pXPG
  • Backbone manufacturer
    Cockerill lab, Addgene plasmid 71248
  • Total vector size (bp) 6766
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GM-CSF promoter -627 to +28 fragment
  • Alt name
    colony stimulating factor 2 (granulocyte-macrophage)
  • Species
    H. sapiens (human)
  • Entrez Gene
    CSF2 (a.k.a. CSF, GMCSF)
  • Promoter GM-CSF
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    human GM-CSF promoter was provided by Judith Gasson and subcloned from the CAT plasmid pCSFp1+ (Chan et al, PNAS 83, 8669-8673, 1986).
  • Terms and Licenses

Depositor Comments

Before being inserted into pXPG, the GM-CSF promoter has been through successive rounds of recloning which have added cloning sites to the ends.

JM109 are the cells used by the author for pXPG and its derivatives.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGM was a gift from Peter Cockerill (Addgene plasmid # 71250 ; http://n2t.net/addgene:71250 ; RRID:Addgene_71250)
  • For your References section:

    The human granulocyte-macrophage colony-stimulating factor gene is autonomously regulated in vivo by an inducible tissue-specific enhancer. Cockerill PN, Bert AG, Roberts D, Vadas MA. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15097-102. PubMed 10611344