Purposeluciferase reporter with human GM-CSF promoter -627 to + 28
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71250||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerCockerill lab, Addgene plasmid 71248
- Total vector size (bp) 6766
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGM-CSF promoter -627 to +28 fragment
Alt namecolony stimulating factor 2 (granulocyte-macrophage)
SpeciesH. sapiens (human)
Entrez GeneCSF2 (a.k.a. CSF, GMCSF)
- Promoter GM-CSF
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Before being inserted into pXPG, the GM-CSF promoter has been through successive rounds of recloning which have added cloning sites to the ends.
JM109 are the cells used by the author for pXPG and its derivatives.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGM was a gift from Peter Cockerill (Addgene plasmid # 71250 ; http://n2t.net/addgene:71250 ; RRID:Addgene_71250)
For your References section:The human granulocyte-macrophage colony-stimulating factor gene is autonomously regulated in vivo by an inducible tissue-specific enhancer. Cockerill PN, Bert AG, Roberts D, Vadas MA. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15097-102. PubMed 10611344