|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74465||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5060
- Total vector size (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namegranulocyte-monocyte colony-stimulating factor
SpeciesM. musculus (mouse)
Insert Size (bp)442
Entrez GeneCsf2 (a.k.a. CSF, Csfg, Csfgm, GMCS, GMCSF, Gm-CS, Gm-CSf, MGI-I, MGI-IGM)
- Promoter CMV
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This plasmid was derived by PCR and T/A cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCR3.1-mGM-CSF was a gift from Loree Heller (Addgene plasmid # 74465 ; http://n2t.net/addgene:74465 ; RRID:Addgene_74465)
For your References section:In vivo electroporation of plasmids encoding GM-CSF or interleukin-2 into existing B16 melanomas combined with electrochemotherapy induces long-term antitumour immunity. Heller L, Pottinger C, Jaroszeski MJ, Gilbert R, Heller R. Melanoma Res. 2000 Dec;10(6):577-83. PubMed 11198480