Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Tandem p40p35
(Plasmid #108665)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108665 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRES-EYFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5163
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    interleukin-12
  • Alt name
    p40
  • Alt name
    p35
  • Species
    M. musculus (mouse)
  • Mutation
    IL-12 dimers have a Gly-Ser linker
  • Entrez Gene
    Il12a (a.k.a. IL-12p35, Il-12a, Ll12a, p35)
  • Entrez Gene
    Il12b (a.k.a. Il-12b, Il-12p40, Il12p40, p40)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRv (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tandem p40p35 was a gift from Nevil Singh (Addgene plasmid # 108665 ; http://n2t.net/addgene:108665 ; RRID:Addgene_108665)