Tandem p40p35
(Plasmid #108665)

• Purpose: Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker
• Depositing Lab: Nevil Singh
• Publication: Nevil Singh IL-12 construct (unpublished)

Backbone

• Vector backbone: pIRES-EYFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5163
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: interleukin-12
• Alt name: p40
• Alt name: p35
• Species: M. musculus (mouse)
• Mutation: IL-12 dimers have a Gly-Ser linker
• Entrez Gene: Il12a (a.k.a. IL-12p35, Il-12a, Ll12a, p35)
• Entrez Gene: Il12b (a.k.a. Il-12b, Il-12p40, Il12p40, p40)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRv (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry