PurposeExpresses tRNA and synthetase for mammalian encoding of a photocaged cysteine
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71404||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6500
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameaminoacyl-tRNA synthetase for a caged cysteine
Insert Size (bp)2500
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Pme I (not destroyed)
- 5′ sequencing primer pCMV (Common Sequencing Primers)
The aminoacyl-tRNA synthetase insert contains ML42GQ compared to all available reference sequences. The depositor states that these mutations are intended and the plasmid functions as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMAH2-CageCys was a gift from Huiwang Ai (Addgene plasmid # 71404 ; http://n2t.net/addgene:71404 ; RRID:Addgene_71404)
For your References section:Light activation of protein splicing with a photocaged fast intein. Ren W, Ji A, Ai HW. J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. Epub 2015 Feb 5. 10.1021/ja508597d PubMed 25647354
Map uploaded by the depositor.