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pMAH2-CageCys
(Plasmid #71404)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71404 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    aminoacyl-tRNA synthetase for a caged cysteine
  • Insert Size (bp)
    2500
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Pme I (not destroyed)
  • 5′ sequencing primer pCMV
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The aminoacyl-tRNA synthetase insert contains ML42GQ compared to all available reference sequences. The depositor states that these mutations are intended and the plasmid functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAH2-CageCys was a gift from Huiwang Ai (Addgene plasmid # 71404 ; http://n2t.net/addgene:71404 ; RRID:Addgene_71404)
  • For your References section:

    Light activation of protein splicing with a photocaged fast intein. Ren W, Ji A, Ai HW. J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. Epub 2015 Feb 5. 10.1021/ja508597d PubMed 25647354