PurposeExpresses suppression tRNA and mCherry inserted with a Npu DnaE intein
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71405||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerlab constructed
- Backbone size w/o insert (bp) 6400
- Total vector size (bp) 7600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNpu DnaE Intein insertion in mCherry
Insert Size (bp)1200
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer pCMV (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMAH2-intein/mCherry was a gift from Huiwang Ai (Addgene plasmid # 71405 ; http://n2t.net/addgene:71405 ; RRID:Addgene_71405)
For your References section:Light activation of protein splicing with a photocaged fast intein. Ren W, Ji A, Ai HW. J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. Epub 2015 Feb 5. 10.1021/ja508597d PubMed 25647354
Map uploaded by the depositor.