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pSRK2706
(Plasmid #78571)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78571 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Express in BL21(DE3) cells
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human rhinovirus 3C protease
  • Species
    H. sapiens (human)
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSRK2706 was a gift from David Waugh (Addgene plasmid # 78571 ; http://n2t.net/addgene:78571 ; RRID:Addgene_78571)
  • For your References section:

    A dual protease approach for expression and affinity purification of recombinant proteins. Raran-Kurussi S, Waugh DS. Anal Biochem. 2016 Apr 19;504:30-37. doi: 10.1016/j.ab.2016.04.006. 10.1016/j.ab.2016.04.006 PubMed 27105777