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PurposeGateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusions
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78572 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMalC2
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 8319
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGateway cloning cassette
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Speciessynthetic
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Insert Size (bp)1800
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSRK2721 was a gift from David Waugh (Addgene plasmid # 78572 ; http://n2t.net/addgene:78572 ; RRID:Addgene_78572) -
For your References section:
A dual protease approach for expression and affinity purification of recombinant proteins. Raran-Kurussi S, Waugh DS. Anal Biochem. 2016 Apr 19;504:30-37. doi: 10.1016/j.ab.2016.04.006. 10.1016/j.ab.2016.04.006 PubMed 27105777
Map uploaded by the depositor.
