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pU6T7G
(Plasmid #71463)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71463 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57KAN
  • Backbone manufacturer
    Genscript
  • Backbone size (bp) 2957
  • Modifications to backbone
    Addition of hybrid human U6/bacteriophage T7 promoter, gRNA cloning site and gRNA sequence
  • Vector type
    Mammalian Expression, Bacterial Expression, CRISPR
  • Promoter human U6/phage T7

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Unknown

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6T7G was a gift from Willie Mark (Addgene plasmid # 71463 ; http://n2t.net/addgene:71463 ; RRID:Addgene_71463)
  • For your References section:

    A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs. Romanienko PJ, Giacalone J, Ingenito J, Wang Y, Isaka M, Johnson T, You Y, Mark WH. PLoS One. 2016 Feb 5;11(2):e0148362. doi: 10.1371/journal.pone.0148362. eCollection 2016. PONE-D-15-48175 [pii] PubMed 26849369